Research on the characteristics of calls from psychological assistance hotline and the coping strategies of operators before and after the outbreak of COVID-19 in Changchun City / 四川精神卫生

ObjectiveTo discuss the characteristics of psychological assistance hotline calls and operators' coping strategies of before and after the COVID-19 outbreak， in order to further improve the assistance ability of the psychological crisis hotline. MethodsA retrospective study was conducted on the demographics characteristics， call problems， coping strategies， and call time trends recorded by Changchun psychological assistance hotline information registration platform before the epidemic in Changchun City （January 20， 2019-April 20， 2019） and during the epidemic period （January 20， 2020-April 20， 2020）. ResultsThe differences between gender， age， marital status， location， and occupation type before and during the epidemic were statistically significant （χ2=11.205， 234.240， 152.083， 265.458， 353.385， P<0.01）. The number of different help calls had a statistically significant difference before and during the epidemic （χ2=185.088，P<0.01）. The difference in the number of operators’ different coping strategies before and during the epidemic was statistically significant （χ2=226.810， P<0.01）. Before the epidemic， the main peak of incoming calls was concentrated at 16∶00 to 17∶00， and the secondary peak was concentrated at 22∶00 to 23∶00. During the epidemic， the main peak of incoming calls was also concentrated at 16∶00 to 17∶00， while the secondary peak was concentrated at 10∶00 to 11∶00. ConclusionDuring the COVID-19 epidemic， the number of calls to the psychological assistance hotline was higher than that before the outbreak. The main peak time for calls was the same， and the secondary peak was adjusted from 22∶00 to 23∶00 to 10∶00 to 11∶00. During the epidemic， the number of calls from male， 30 to 39 years old， married， local and staff in Changchun was the most， psychological problems counseling and operator referral strategy were the most before and after the epidemic.

Correlation of HBV genotyping and drug resistance mutation in Hohhot,Inner Mongolia / 中国基层医药

Objective To investigate the relationship between HBV -DNA load and serum markers in chronic hepatitis B( CHB) patients in Hohhot,Inner Mongolia,and to explore the mutation of HBV genotype and nucleoside analogue.Methods From January 2015 to December 2017,one hundred and ninety-three CHB patients hospitalized in the People＇s Hospital of Inner Mongolia were selected randomly.The clinical diagnostic criteria for all admitted patients were based on the " Guidelines for the Prevention and Treatment of Chronic Hepatitis B" jointly formulated by the Infectious Diseases Society of 2010. The HBV -DNA load of HBV was detected by real -time quantitative PCR,and the correlation between HBV -DNA load and serum markers was analyzed. Seventy -nine patients were selected from 193 hospitalized patients,PCR-reverse dot blot hybridization was used to analyze HBV genotyping and the drug resistance mutations of different genotypes.Results The differences of HBeAb level and HBV-DNA load between HBeAg positive patients and negative patients were statistically significant(all P<0.001). Of 79 serum specimens of HBV infected people,9 cases(11.4% ) were B genotypes,and 70 cases of C genotype (88.6% ).Of them,25 cases had different loci variation,the rate of variation was 31.6% (25/79),with the unit point rtS213T mutation dominated,accounting for about 24.0% (6/25).Conclusion In Hohhot Inner Mongolia patients with CHB,HBV-DNA load with HBeAg and HBe Ab level are correlated;genotype in patients including B type and C type,which is mainly genotype C;patients with CHB mainly had drug resistance to lamivudine and adefovir dipivoxil, mutations including rtS213T,and hybrid mutation.

Chemical constituents from the rhizomas of Smilax glauco-china / 中成药

AIM To study the chemical constituents from the rhizomas of Smilax glauco-china Warb.METHODS The n-butanol fraction of ethanol extract of S.glauco-china was isolated and purified by silica,Sephadex LH-20 and semi-preparative column,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Ten compounds were isolated and identified as phenethanol-β-D-gentiobioside (1),2-phenylethyl-O-β-D-xylopyranosyl-(1 →6)-β-D-glucopyranoside (2),phenylethyl D-rutinoside (3),phenylethyl β-D-glucoside (4),hydrangeifolin Ⅰ (5),icariside D1 (6),calophymembranside B (7),2-hydroxyphenol-1-O-β-D-glucopyranosyl-(6 → 1)-α-L-rhamnopyranoside (8),β-sitosterol (9),daucosterol (10).CONCLUSION All the compounds are isolated from this plant for the first time.

Analysis on Development Modes of Internet Medical Treatment in China / 医学信息学杂志

By literature and network investigation,the paper sorts out development modes and typical practices of Internet medical treatment in China,sums up 6 kinds of modes existing domestically,analyzes the advantages and disadvantages of each mode.It is necessary to further probe into suitable operating modes and to give support on aspects of policies,laws and regulations and supervision,and provides reference for better promoting Internet medical treatment in different places.

Detection of antimicrobial resistance and OXA genes in imipenem-resistant Acinetobacter baumannii from Hohhot region / 中国感染控制杂志

Objective To realize antimicrobial resistance and carrying status of OXA carbapenemase among imi-penem-resistant Acinetobacter baumannii (IRAB)isolated from patients of Hohhot,so as to provide guidance for the prevention and control of healthcare-associated infection(HAI)caused by multidrug-resistant Acinetobacter bauman-nii .Methods 49 IRAB isolates from 3 tertiary first-class hospitals in Hohhot between January and December 2012 were collected,antimicrobial susceptibility testing was performed by Kirby-Bauer disk diffusion method,four geno-types(blaOXA-51-like ,blaOXA-23-like ,blaOXA-24-like ,blaOXA-58-like )of OXA carbapenemase were detected by polymerase chain reaction (PCR).Results All 49 isolated IRAB strains were found to be highly resistant to antimicrobial agents (81 .63%-100.00%)except to minocycline (8.16%);blaOXA-51-like was identified in 49 strains (100.00%),42 (85.71 %)of which also carried blaOXA-23-like gene ,blaOXA-23-lik and blaOXA-51-like were both found in three hospital, blaOXA-24-like and blaOXA-58-like weren’t found.Conclusion IRAB strains present multidrug resistance,resistant to mi-nocycline is the lowest;blaOXA-23-like is the main drug-resistance mechanism of IRAB in Hohhot.

Study on risk factors with the occurrence of the mesh exposure after pelvic floor reconstruction / 中华妇产科杂志

Objective To study risk factors with the occurrence of the mesh exposure after pelvic floor reconstruction.Methods From Mar.2007 to Mar.2011,a retrospective study was made on the clinical data of 353 patients undergoing vaginal mesh pelvic floor reconstruction.The related complications of the mesh were surveyed,and risk factors associated with the mesh's exposure were studied by single factor and multiple factors logistic regression.Results It was found that the exposure rate was 9.6％ (34/353) after 1 year postoperative follow-up.Single factor analysis showed that patients who were not less than 70 years old,patients who were on their menopause stage,the occurrence of not less than three parturition,history of pelvic surgery,diabetes and smoking had a significant correlation (all P ＜ 0.05) with the occurrence of the mesh exposure after pelvic floor reconstruction.Multiple factors logistic regression analysis showed that patients who were not less than 70 years of age (OR =2.389),the occurrence of not less than three parturition (OR =2.688),the history of diabetes (OR =3.545),the history of pelvic surgery (OR =5.385) were the independent risk factors,and the operation experience was the protection factors (OR =0.134).Conclusions Mesh exposure is a common complication after pelvic floor reconstruction.Old age,multiple delivery,history of diabetes and history of pelvic surgery are the risk factors of mesh exposure.Preoperative full assessment and the quality of training contribute to reduce the incidence.

Effective management and prevention of septic shock after minimally invasive percutaneous nephrolithotomy in the elderly / 中华老年医学杂志

Objective To explore the cause and effective management and prevention of septic shock after minimally invasive percutaneous nephrolithotomy (MPCNL) in the elderly.Methods Clinical data of 13 patients with septic shock after MPCNL in our hospital were retrospectively analyzed,and the cause,prevention and treatment of septic shock were investigated.Results The incidence of septic shock in patients undergoing MPCNL was 3.54％ in this study.Single-factor analysis and logistic regression analysis showed that the risk factors for post-MPCNL septic shock were female and the history of diabetes (both P＜0.05).Conclusions Septic shock is a serious complication after MPCNL.Intensive perioperative prevention measures can reduce the incidence of septic shock.Early diagnosis and proper treatment are the keys to successful treatment of septic shock.

S100B Protein and Central Nervous System Injury / 国际脑血管病杂志

S100B protein has a higher sensitivity to central nervous system(CNS),which can be used in clinical and prognostic prediction of various forms and different degrees of CNS injury,even in the judgment of brain death.The measurement of S100B protein and prediction of secondary brain injtry enable physicians to select an appropriate opportunity for therapy intervention,which will be a focus in the future studies.S100B protein is a potential protein biomarker that has clinical prospect.

Research and implementation of picture archiving and communication system (PACS) based on B/S mode / 生物医学工程学杂志

In this paper, with B/S application and architecture, an integrated solution of PACS is designed, and the function and application of each part of PACS based on the architecture is introduced. The PACS based on this mode is safe, stable, easy to manage and upgrade and convenient for use in telemedicine.

Correlation of orbital computed tomography and incretion in patients with thyroid associated ophthalmopathy-Ⅰ / 医学研究生学报

Objective:To evaluate the correlation of orbital computed tomography and incretion in patients with thyroid associated ophthalmopathy- Ⅰ. Methods : Seventy-six orbits of 38 patients with thyroid associated ophthalmopathy were evaluated by CT in respect to the density and the size of extraocular muscles, and 100 normal orbits of 50 healthy person were evaluated with the same indices for comparison. Results:There was significant difference in the density and the size of extraocular muscles between the patients and the healthy adults. The maximal size of extraocular muscles were positive correlation with T3,T4in TAO-Ⅰ, and negative correlation with TSH. Conclusion:The manifestation of orbital CT may be a good index in the following survey of thyroid associated ophthalmopathy-Ⅰ.

Hypervascular Hepatocellular Carcinoma:The Detection of Multi-slice Spiral CT,Digital Subtraction Angiography and Lipiodol CT / 实用放射学杂志

20 mm),these three imaging techniques(HAP-MDCT,Lipiodol CT and DSA)had the same sensitivity,for 10~20 mm hepatic nodules,the detecting rate was no significant difference among them,while,for

?_2-AR gene cloning from human detrusor cell and the construction of its antisense eukaryotic expression vector / 中华泌尿外科杂志

Objective To clone human beta-2 adrenoceptor gene from human bladder smooth muscle and to construct its antisense eukaryotic expression vector. Methods The ?_2-AR full length cDNA was cloned from human detrusor cells through RT-PCR and subcloned into clone vector (pUC18).The objective gene was then cut from ClaⅠ/HindⅢ sites of pUC18 with restriction endonulcease and subcloned into pLNCX vector in trans-direction.Finally the constructed ?_2-AR gene antisense expresstion vector was identified through restriction endonuclease analysis and sequencing. Results The sequence of cloned ?_2-AR full length cDNA was certified by comparison with the database of the Genebank.The constructed antisense eukaryotic expression vector was proved to be same with designed by restriction endonuclease analysis and sequencing. Conclusions ?_2-AR full length cDNA was cloned and its antisense eukaryotic expression vector was successfully constructed.This technique establishs the foundation for the further research on drug treatment of bladder dysfunction.

EFFECTS OF SOYBEAN PHOSPHOLIPIDS ON CONTENTS OF LIPIDS IN ERYTHROCYTE MEMBRANES OF MICE / 营养学报

Objective: To study the effects of soybean phospholipids on fatty acids and phospholipids content in erythrocyte membranes of mice. Methods:The mice were fed with soybean phospholipids of 2 . 5, 5 . 0 , 10.0 g/kg bw respectively while control group with distilled water for 30 days. Then the mice were killed and contents of fatty acids and phospholipids in erythrocyte membranes were detected by HPLC. Results: (1)The contents of linoleic acid (C18 ∶2) and linolenic acid ( C18 ∶3), the composition of polyunsaturated fatty acid (PUFA) were increased obviously (P